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. 2018 Mar 8;16(3):83. doi: 10.3390/md16030083

Table 2.

Comparison of chitinolytic enzyme, protease and αGI production by various bacteria using SPP as C/N source.

Strain Activity (U/mL)
Exochitinase Chitinase Chitosanase Protease aGI
Paenibacillus macerans TKU029 - 0.376 + 0.026 0.857 + 0.015 0.135 ± 0.011 60.13 ± 6.844
P. mucilaginosus TKU032 - 0.101 + 0.017 0.155 + 0.016 0.193 ± 0.005 175.40 ± 0.606
Paenibacillus sp. TKU037 - 0.105 + 0.018 0.247 + 0.014 0.222 ± 0.004 175.80 ± 0.851
Paenibacillus sp.TKU042 - 0.185 + 0.019 0.928 + 0.014 0.199 + 0.005 88.30 ± 4.502
Bacillus licheniformis TKU004 1.211 + 0.017 0.074 + 0.017 0.120 + 0.014 0.707 + 0.020 174.59 ± 4.295
Bacillus subtilis TKU007 - 0.118 + 0.017 0.314 + 0.017 0.219 + 0.005 58.64 ± 7.368
Lactobacillus paracasei TKU010 - - 0.166 + 0.015 0.090 + 0.010 -
Serratia marcescens TKU011 8.039 + 0.296 0.177 + 0.018 0.150 + 0.013 0.477 + 0.010 -
Serratia ureilytica TKU013 11.545 + 0.431 0.319 + 0.019 0.112 + 0.013 0.910 + 0.031 52.60 ± 8.418
Pseudomonas tamsuii TKU015 - 0.105 + 0.015 0.056 + 0.013 0.113 + 0.009 -
Serratia sp. TKU016 - 0.111 + 0.018 0.302 + 0.012 0.370 + 0.012 -
Serratia sp. TKU020 9.327 + 0.325 0.201 + 0.018 0.118 + 0.014 0.478 + 0.021 52.49 ± 0.778
Bacillus cereus TKU028 - 0.223 + 0.018 0.520 + 0.015 0.153 + 0.007 68.64 ± 3.972
Bacillus mycoildes TKU038 - 0.332 + 0.019 0.611 + 0.016 0.240 + 0.004 71.46 ± 3.635
Bacillus mycoildes TKU040 - 0.350 + 0.016 0.165 + 0.013 0.083 + 0.011 -
Rhizobium sp.TKU041 - - - 0.198 + 0.012 -

SPP was used at the sole C/N source with a concentration of 1% (w/v). Culture conditions were set at 37 °C, 150 rpm shaking speed, 100/250 mL (volume of medium/flask) for two days. The cultures of fermented deCSP were obtained and centrifuged at 12,000× g for 20 min to collect the culture supernatants. (-) expressed activity lower than 0.1 U/mL and 10 U/mL for exochitinase and αGI, respectively.