Fig. 5.

ω-6 supplementation increases proinflammatory lipid mediators with no structural changes in the left ventricle (LV). A: schematic representation of targeted liquid chromatography-mass spectrometry analyses (LC/MS) analysis. B: quantitative analysis of 12(S)-HETE, 15(S)-HETE, leukotriene B₄ (LTB₄), PGE₂, and thromboxane B₂ (TXB₂) in young and aging mice fed with standard laboratory chow (LC) or excess ω-6 diet. Red, young-LC group; black, young-safflower oil (SO) group; purple, aging-LC group; green, aging-SO group. C: multiple reaction monitoring (MRM) peaks of 12(S)-HETE in young and aging mice with and without ω-6 supplementation. D: structure of 12(S)-HETE. E: representative wheat germ agglutinin (WGA) membrane and Hoechst nuclear-stained images showing LV cardiomyocytes from young and aging mice fed with LC or ω-6-enriched diets. F: 5-lipoxygenase (5-LOX) methylation in aging by bisulphite PCR. Values are means ± SE; n = 5 mice/group. *P < 0.05 as analyzed by one-way ANOVA.