Fig. 6.
Supplementation of excess ω-6-in aging disrupts nuclear erythroid 2-related factor 2 (Nrf2)-mediated antioxidant signaling. A−C: mRNA expression of Nrf2, Kelch-like ECH associated protein 1 (Keap-1), glutathione peroxidase 1 (GPX-1), and glutathione-S-transferase-α4 (Gsta4) (A), NAD(P)H quinone dehydrogenase 1 (Nqo-1), glutathione-disulfide reductase (GSR), glutamate-cysteine ligase catalytic subunit (Gclc), glutamate-cysteine ligase modifier subunit (Gclm) (B); and glucose-6-phosphate dehydrogenase (G6PD), catalase (Cat), superoxide dismutase (SOD)1, and SOD2 (C) in young and aging mice fed with standard laboratory chow (LC) or excess ω-6 diet. Values are means ± SE; n = 4 mice/group. *P < 0.05 vs. the young-LC group; $P < 0.05 LC vs. safflower oil (SO).