Table 1.

Summary of published studies using the WS in SSc.

Study	Saliva sample	Patients/controls	Analytical methods	Findings
Giusti et al. [14]	UWS	15/15	2-DE, MALDI-TOF/MS	Presence of 9 proteins only in SSc (calgranulin A, calgranulin B, psoriasin, Arp2/3 complex, ß2-microglobulin, TPI, GAPDH, cyclophilin A, and cystatin B).
Baldini et al. [13]	UWS	44/80	SDS/PAGE, Western blot	Significant association of psoriasin with pulmonary involvement in dcSSc.
Knaś et al. [7]	UWS/SWS	97/55	ELISA, Spectrophotometrically	(i) In UWS of dcSSc and lcSSc:  (1) Salivary flow, the output of total protein, and peroxidase activity were significantly lower.  (2) sIgA and lactoferrin were significantly higher. (ii) In SWS:  (1) In lcSSc, the total lysozyme and peroxidase activity were significantly higher.  (2) In dcSSc, the salivary flow was significantly lower and the total sIgA and peroxidase activity were significantly higher.
Giusti et al. [12]	UWS	134/74	ELISA	Significant correlation between salivary psoriasin and DLCO in SSc.

dcSSc, diffuse cutaneous systemic sclerosis; DLCO, diffusion capacity of carbon monoxide; ELISA, enzyme-linked immunosorbent assay; lcSSc, limited cutaneous systemic sclerosis; MALDI-TOF, matrix-assisted laser desorption ionization; MS, mass spectroscopy; SDS/PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; SSc, systemic sclerosis; SWS, stimulated whole saliva; UWS, unstimulated whole saliva; 2-DE, two-dimensional gel electrophoresis.