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. 2018 Mar 13;9(3):162. doi: 10.3390/genes9030162

Figure 4.

Figure 4

Effect of cell-wall-perturbing agents calcofluor white (CFW) and Congo red (CR) on growth of Vd-wt, ΔVdPLP (ΔVdPLP-1 and ΔVdPLP-4) strains, and complementary ΔVdPLP (ΔVdPLP-1-C and ΔVdPLP-4-C) strains. (A) Colonies of Vd-wt, ΔVdPLP (ΔVdPLP-1 and ΔVdPLP-4) mutant strains, and complementary ΔVdPLP mutant (ΔVdPLP-1-C and ΔVdPLP-4-C) strains after 15 days on potato dextrose agar (PDA) plates containing 100 g/L CR or 50 g/L CFW compared with controls on PDA at 25 °C. (B) Mean (± SD) colony diameter after 15 days on PDA plates with 100 g/L CR or 50 g/L CFW compared with controls on PDA. Three independent replicates were done; Different letters (a–c) above the bars represent the significant differences among the groups and were analyzed with the Duncan’s multiple range test (p-value < 0.05).