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Fungal colonization and penetration assay: (A) Vd-wt, ΔVdPLP and ΔVdPLP-C strains grown on cellophane for three days after strains had penetrated a cellophane membrane on the agar plate and membranes were removed; (B) plates after incubation at 25 °C for seven days after cellophane were removed; and (C) fungal colonization of infected roots by the ΔVdPLP-4-GFP mutant strains, Vd-GFP strain, and sterile water (Mock), three days after inoculation.
