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. 2018 Mar 13;9(3):162. doi: 10.3390/genes9030162

Figure 7.

Figure 7

ΔVdPLP mutant strains were not virulent on Nicotiana benthamiana in pathogenicity assays. (A) Representative plants 12 days post inoculation (dpi) with Vd-wt, ΔVdPLP strains (ΔVdPLP-1 and ΔVdPLP-4), or complementary ΔVdPLP strains (ΔVdPLP-1-C and ΔVdPLP-4-C); (B) Disease level on N. benthamiana after a 2-min root-dip in conidia of the Vd-wt, ΔVdPLP strains (ΔVdPLP-2 and ΔVdPLP-4), and complementary ΔVdPLP mutant (ΔVdPLP-1-C and ΔVdPLP-4-C) strains at 8, 10, and 12 dpi. See methods for severity scale; (C) Relative quantification of fungal biomass in N. benthamiana stems inoculated with conidial suspension of Vd-wt, ΔVdPLP strains (ΔVdPLP-1 and ΔVdPLP-4), and complementation ΔVdPLP strains (ΔVdPLP-1-C and ΔVdPLP-4-C) at 12 dpi. Values are means ± SD from three independent replicates. Significant differences are indicated by letters (a–d), Duncan’s multiple range test (p-value < 0.05) was used to analyze significant difference between each group.