Table 1.

Primers used in this study. Under lines indicate the attB sequence.

Primer	Primer Sequence, 5′→3′
VeA-up-s	GGGGACAGCTTTCTTGTACAAAGTGGAAGGGTAGAACGGGACGACT
VeA-up-as	GGGGACTGCTTTTTTGTACAAACTTGTGCGGGAAGGGTAAGTGAG
VeA-down-s	GGGGACAACTTTGTATAGAAAAGTTGTTCCACCTATTGTTCGCTTGA
VeA-down-as	GGGGACAACTTTGTATAATAAAGTTGTGTCCTGGGCATCCTTGTT
Ura3(s)	GTCAAGACATCTGTTACCGTGG
Ura3(as)	GCAGGCGGGTAGTAGAGT
VeA(s)	GGGGACAGCTTTCTTGTACAAAGTGGAAGGGTAGAACGGGACGACT
VeA(as)	GGGGACAACTTTGTATAATAAAGTTGTCGACGGAAGCCTGTATCAA
Hyg(s)	CCGGTCGGCATCTACTCT
Hyg(s)	CGTTGCAAGACCTGCCTGAA
ART(F)	CATTCACAAGGCGGGAGA
ART(R)	CGAACAATAGGTGGAGGGTC
VelB-up-s	GGGGACAGCTTTCTTGTACAAAGTGGAAACGACGGTTGTGGTTCAG
VelB-up-as	GGGGACTGCTTTTTTGTACAAACTTGTAGGGCAATCCAGGTAAGC
VelB-down-s	GGGGACAACTTTGTATAGAAAAGTTGTTAGGTTATGATACAATCGGGTTA
VelB-down-as	GGGGACAACTTTGTATAATAAAGTTGTAGACAAGAGGTGCGGAAA
VelB(s)	CGCACAGCCCATTTAGAG
VelB(as)	CCAAGCCATCAGATCGTG
BRT(F)	GATGGAACCTGGACATACAA
BRT(R)	ACGGAGGAGGCGTGATAG
LaeA-up-s	GGGGACAGCTTTCTTGTACAAAGTGGAAAACCCTCCACCTCAACAACA
LaeA-up-As	GGGGACTGCTTTTTTGTACAAACTTGTTAGCAAGCCAATACACGATG
LaeA-down-s	GGGGACAACTTTGTATAGAAAAGTTGTTGGTTGCCCTTGAGCCTTGAA
LaeA-down-as	GGGGACAACTTTGTATAATAAAGTTGTCGCCGTATAAATGTACCTTGC
LaeA(s)	GGGGACAGCTTTCTTGTACAAAGTGGAATGCCAGGTTGTTCAGTAA
LaeA(as)	GGGGACAACTTTGTATAATAAAGTTGTGAGTCGGGCGGGTAGATT
LRT(F)	ACCCTCCACCTCAACAAC
LRT(F)	CATTAGCAAGCCAATACAC
Actin1(s)	GTCGCTGCCCTCGTTATC
Actin1(as)	CGAGAATGGAACCACCGAT