Figure 2.

Ron promotes homeostasis in the central nervous system (CNS). (A) Weight of wild-type (WT) and Ron^−/− mice at 27 weeks of age (n = 16 mice/genotype). (B,C) Expression of pro-inflammatory genes and MMPs in the CNS of WT and Ron^−/− at 27 weeks of age (n = 6–8 mice/genotype). (D,E) Immune cells were isolated from the CNS of WT and Ron^−/− mice at 27 weeks and the presence of CD45⁺F480⁺CD206⁺ macrophages was assessed by flow cytometry (n = 4 mice/genotype). (F) OPLS-DA scores plot (left) and coefficient plot (right) derived from ¹H NMR spectra of CNS samples from WT and Ron^−/− mice at 27 weeks of age. (G) Relative abundance of CNS metabolites measured by ¹H NMR data from WT and Ron^−/− mice at 27 weeks of age (n = 6 mice/genotype) Abbreviations: BCAAs, branched chain amino acids; ala, alanine; met, methionine; glu, glutamate; gln, glutamine; tyr, tyrosine; phe, phenylalanine; his, histidine; MMPs, matrix metalloproteinases (*P < 0.05, **P < 0.01, ***P < 0.001).