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. 2018 Mar 19;9:513. doi: 10.3389/fimmu.2018.00513

Figure 4.

Figure 4

High fat diet-induced obesity promotes inflammation in the central nervous system (CNS). (A) Weight of mice following 18 weeks on a HFD or chow controls (n = 18 mice/group). (B) A terminal intra-peritoneal glucose tolerance test was performed on mice following 18 weeks on a HFD or chow controls (n = 6 mice/group). (C) Representative body morphology of mice following 18 weeks on a HFD or chow controls. (D) Circulating leukocyte counts from mice following 18 weeks on a HFD or chow controls (n = 10–12 mice/group). (E–G) Mice were maintained on a HFD or chow for 18 weeks and expression of M1-associated genes, M2-associated genes and matrix metalloproteinases (MMPs) in the CNS was assessed by qPCR (n = 12 mice/group). (H,I) Total CNS immune cells were isolated from mice maintained on a HFD or chow for 18 weeks and the number of CD45+F4/80+Ly6C+ infiltrating monocyte/macrophages was assessed by flow cytometry (n = 6 mice/group) (*P < 0.05, **P < 0.01, ***P < 0.001).