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Fig. 2. (a) Dose–dependent curves for cell viability of HeLa cells treated with Ru1 and Ru2 using a typical MTT assay under light irradiation (L) or in the dark (D). Cells were irradiated with white light (400–800 nm, 30 mW cm–2, 10 min). (b) Flow cytometric assay of fluorescence intensity of DCF, cells incubated with Ru(ii) complexes (5 μM) were treated with light irradiation (35 mW cm–2). (c and d) Confocal fluorescence images of ROS generation in cells incubated with Ru(ii) complexes (5 μM), cells were treated with light irradiation (400–800 nm, 35 mW cm–2) for 10 min. The images share the same scale bar of 50 μm.