Table 2.
Antibodies (Materials And Methods).
| Antibody | Immunogen | Source (Cat. No.), species | RRID | Dilution |
|---|---|---|---|---|
| PRIMARY | ||||
| GFP | Green Fluorescent Protein | Life technologies (A10262), chicken (monoclonal) | AB2534023 | 1:400 |
| TH | Tyrosine Hydroxylase from zebrafish th1 gene fragment | (Ryu et al., 2007), rabbit (polyclonal) | AB2631248 | 1:500 |
| Islet1/2 | Recombinant fusion protein containing aa 178–349 of Isl1 protein (C-terminus) | DSHB (39.4D5), mouse (monoclonal) | AB2314683 | 2 μg/ml |
| SECONDARY | ||||
| Alexa 488 | Chicken IgG | Life technologies (A11039), Goat | AB2534096 | 1:1,000 |
| Alexa 555 | Rabbit IgG | Life technologies (A21430), Goat | AB2535851 | 1:1,000 |
| Alexa 555 | Mouse IgG | Life technologies (A21425), Goat | AB2535846 | 1:1,000 |
| Alexa 633 | Rabbit IgG | Life technologies (A21070), Goat | AB2535731 | 1:1,000 |