Table 5.
Larvae analyzed for | Lateral line organs analyzed | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ANTERIOR LATERAL LINE | |||||||||||||
SO | N1 | N2 | M1 | M2 | SO1 | SO2 | SO3 | IO1 | IO2 | IO3 | IO4 | O1 | O2 |
GFP pos. | 9 | 9 | 7 | 4 | 11 | 12 | 12 | 4 | 4 | 4 | 4 | 12 | 12 |
TH ir | 9 | 9 | 7 | 4 | 10 | 11 | 12 | 4 | 4 | 4 | 4 | 12 | 12 |
POSTERIOR LATERAL LINE (MEDIAL AND DORSAL BRANCHES) | |||||||||||||
SO | MI1 | MI2 | OP | D1 | D2 | D3 | D4 | D5 | |||||
GFP pos. | 11 | 11 | 5 | 11 | 14 | 8 | 8 | 5 | |||||
TH ir | 11 | 11 | 5 | 10 | 13 | 8 | 7 | 4 | |||||
POSTERIOR LATERAL LINE (LATERAL BRANCHES) | |||||||||||||
SO | L1 | L2 | L3 | L4 | L5 | L6 | L7 | L8 | LII.1 | LII.2 | LII.3 | LII.4 | |
GFP pos. | 8 | 7 | 4 | 5 | 3 | 4 | 2 | 2 | 5 | 3 | 2 | 1 | |
TH ir | 8 | 7 | 5 | 5 | 3 | 4 | 2 | 2 | 6 | 3 | 2 | 1 |
A total number of 32 scans were analyzed and checked for GFP positive innervation (GFP pos.) and TH-immunoreactivity (TH ir). Scans were made from different body regions and with varying orientation of the larvae. 19 scans were made with th:Gal4 larvae expressing UAS:eGFP, 10 th:Gal4 larvae expressed UAS:eGFP-CAAX and additionally UAS:SypGFP, and 3 th:Gal4 larvae expressed only UAS:SypGFP. Discrepancies between TH-immuno-labeling and GFP label likely stem from variegated GFP expression.