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. 2018 Feb 27;9(6):929–940. doi: 10.7150/jca.23042

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Expression levels of CXCR4 and CXCR7 in FTC cell line TT2609-C02. (A) FTC cell line TT2609-C02 exhibits both chemokine receptors CXCR4 and CXCR7. Immunocytochemical staining of CXCR4 and CXCR7 was conducted by using Alexa Fluor® 488 as secondary antibody and DAPI for visualization of the nucleus. Compositions of both images are illustrated as overlays. Isotype controls were used to confirm antibody specificity (Control). Images were captured using a fluorescence microscope at 400x magnification. Bar at the top left corner indicates 50 µm. (B) FTC cell line TT2609-C02 demonstrates a strong protein expression of both chemokine receptors as displayed in the western blot analysis. GAPDH served as loading control. CXCR4/7: C-X-C chemokine receptor type 4/7; DAPI: 4′,6-diamidin-2-phenylindol; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.