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. 2018 Feb 27;9(6):929–940. doi: 10.7150/jca.23042

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Rh-SDF1α induces tumour cell invasion, cell cycle activation and EMT. (A) Pictures display representative results of the change of invading cells after incubation of human FTC cell line TT2609-C02 with different concentrations of CXCR4 antagonists AMD3100 and WZ811 as well as rh-SDF1α as receptor agonist. Cells were visualized by DAPI staining. (B) Invading cells were counted in five visual fields of at least three different membranes. Differences after treatment are illustrated as fold change to control. (C) FACS after PI staining revealed cell cycle changes in the respective cell populations after rh-SDF1α treatment. Cells are grouped according to their specific cell cycle phase. (D) After incubation with rh-SDF1α, mRNA expression changes of genes associated with EMT were investigated using qRT-PCR. GAPDH functioned as housekeeping gene. The 2^-∆∆CT method was employed to estimate fold changes to control treated cells. Plots display the mean +SEM. Numerical data were analysed using the non-parametric Mann-Whitney U test. Ctrl: control; *p<0.05; **p<0.01; ***p<0.001.