Skip to main content
. 2018 Feb 28;9(6):1050–1056. doi: 10.7150/jca.23388

Figure 1.

Figure 1

RhoA inhibitor induced ESCCs apoptosis. (A)Inhibitory effects of fasudil on the RhoA pathway in EC109 and KYSE150 cells. Cells were seeded in a 6-well plate; when the cell growth reached approximately 80% intensity, diverse concentrations of fasudil were added to the culture medium and incubated for 24 h. The activity of RhoA was detected by RhoA activation assay. The results are representative of three different experiments. (B)Characteristic apoptosis was seen in KYSE150 and EC109 cells treated with fasudil for 48 h. (Original magnification, 200×). (C) Western blot analysis was performed with cleaved caspase-3 antibody and caspase-9 antibody probes. Blank: PBS treatment. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.