Skip to main content
. 2018 Feb 28;9(6):1050–1056. doi: 10.7150/jca.23388

Figure 4.

Figure 4

Effect of inhibition of Beclin 1 or ATG 7 expression on fasudil-induced apoptosis in ESCC cells. (A) Western blot showing a decrease in the expression of Beclin 1(or ATG7, figure 4C) when EC109 and KYSE150 cells were transfected with Beclin 1 targeting siRNA vs negative control siRNA. (B) Western blot indicating that silence of Beclin 1 (or ATG 7, figure 4D) with siRNA impeded fasudil-induced raise in LC3-II levels in EC109 and KYSE150 cells. (E) Fasudil combined with autophagy inhibitor effectively induced ESCC cells apoptosis. Combination therapy with fasudil (75 nM×48 h) and chloroquine (5 mM×48 h) led to more ESCC cells apoptosis, higher caspase-3 and caspase-9 activities than fasudil only (*P<0.05, fasudil plus Beclin 1(or ATG 7) siRNA vs fasudil).