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. 2018 Feb 8;293(12):4277–4288. doi: 10.1074/jbc.RA118.002006

Figure 1.

Figure 1.

Acyl acid and amino acid screening for AtGH3.15 activity. A, acyl acid activity screen of AtGH3.15 with each phytohormone (2.5 mm) at invariant ATP (1 mm) and glutamine (25 mm) concentrations. B, amino acid screen of AtGH3.15 with each amino acid (10 mm) at invariant IBA (1 mm) and ATP (1 mm) concentrations. C, amino acid screen of AtGH3.15 with each amino acid (10 mm) and invariant JA (1 mm) and ATP (1 mm) concentrations. All assays were performed using a coupled assay system as described under “Experimental procedures.” Average values ± S.D. (error bars) (n = 3) are shown.