Figure 1.

Cl⁻ channel blockers inhibit the metastatic phenotypic changes associated with ST expression. A and B, in (A) i293-EGFP and i293-EGFP-ST cells were induced with doxycycline hyclate and in (B) MCC13 cells were transfected with EGFP and EGFP-ST. (i) cells were then treated with DMSO, DIDS, RIAA, or NPPB and after 24 h, cell motility was measured using the IncuCyte kinetic imaging system. Images were taken every 30 min for a 24-h period. (ii) scatter plot showing cell movement tracked using ImageJ (n = 25). Average cell movement, as indicated by the horizontal bar, was calculated and significance tested using a two-tailed Student's t test; *, p < 0.001. (iii) After 24 h, inhibitors were washed off cells and media added. Cells were imaged for a further 24 h and average movement calculated (n = 25); *, p < 0.001. C, i293-EGFP and i293-EGFP-ST cells were induced and treated with DMSO, DIDS, RIAA, or NPPB. After 24 h, cells were transferred into migration wells and inhibitors added at appropriate concentrations to allow cells to migrate from serum-free to 10% FBS conditions for 24 h. Migratory cells were stained and measured at 560 nm to quantify migration. Average cell migration was calculated and significance tested using a two-tailed Student's t test (n = 3); *, p < 0.001.