Skip to main content
. 2018 Feb 8;293(12):4582–4590. doi: 10.1074/jbc.RA117.001343

Figure 3.

Figure 3.

CLIC1 and CLIC4 KD inhibits MCPyV ST-induced cell motility. A and B, in A(i) i293-EGFP and i293-EGFP-ST cell lysates or (B)(i) EGFP or EGFP-ST-transfected MCC13 cells were probed for successful knockdown with CLIC1- and CLIC4-specific antibodies. GAPDH was used as loading control and 2T2 hybridoma was used to show MCPyV ST expression. A(ii), i293-EGFP and i293-EGFP-ST cells were transfected with scrambled or CLIC1 and/or CLIC4 siRNA for 12 h, then induced using doxycycline for 24 h. B(ii), MCC13 cells were transfected with scrambled or CLIC1 and/or CLIC4 siRNA for 12 h, then retransfected with EGFP and EGFP-ST after a further 12 h. 48 h post knockdown, cell motility was measured using the IncuCyte kinetic imaging system. Images were taken every 30 min for a 24-h period. C, scatter plots showing cell movement of (i) i293 or (ii) MCC13 cells tracked using ImageJ (n = 25). Average cell movement, indicated by horizontal bar, was calculated and significance was tested using a two-tailed Student's t test. *, p < 0.001.