Table 2.
Cysteine modifications observed in human ERα (P03372)
Tandem mass spectra are provided in the supporting information. Dha, dehydroalanine.
| Residues | Cys positions | Site of S–GSH | All modifications | Peptide sequence | MS/MS | Precursor m/z (MS/MS) | z | Enzyme |
|---|---|---|---|---|---|---|---|---|
| 184–206 | 185, 188, 202, 205 | Sulfide (Cys); Cys to Dha (Cys) | YCAVCNDYASGYHYGVWSCEGCK | CID | 1288.48 | 2 | Trypsin | |
| 190–206 | 202, 205 | Sulfide (Cys); Cys to Dha (Cys) | DYASGYHYGVWSCEGCK | ETD | 641.59 | 3 | Lys-C, Asp-N | |
| 212–231 | 221, 227 | Sulfide (Cys); Cys to Dha (Cys); trioxidation (Cys) | SIQGHNDYMCPATNQCTIDK | CID | 1127.47 | 2 | Trypsin | |
| 212–233 | 221, 227 | 221 | S-Glutathione (Cys); Cys to Dha (Cys) | SIQGHNDYMCPATNQCTIDKNR | ETD | 696.05 | 3 | Trypsin |
| 235–241 | 237, 240 | Sulfide (Cys); Cys to Dha (Cys) | KSCQACR | CID | 397.17 | 2 | Trypsin | |
| 245–252 | 245 | 245 | S-Glutathione (Cys) | CYEVGMMK | ETD | 422.49 | 2 | Trypsin |
| 245–252 | 245 | 245 | S-Glutathione (Cys); oxidation (Met) | CYEVGMMK | ETD | 427.82 | 2 | Trypsin |
| 417–434 | 417 | 417 | S-Glutathione (Cys) | CVEGMVEIFDMLLATSSR | CID | 1154.01 | 2 | Trypsin |
| 417–434 | 417 | 417 | S-Glutathione (Cys); oxidation (Met) | CVEGMVEIFDMLLATSSR | ETD | 769.68 | 3 | Trypsin |
| 437–449 | 447 | 447 | S-Glutathione | MMNLQGEEFVCLK | CID | 923.89 | 2 | Trypsin |
| 437–449 | 447 | 447 | S-Glutathione (Cys); oxidation (Met) | MMNLQGEEFVCLK | ETD | 621.6 | 3 | Trypsin |
| 530–548 | 530 | Trioxidation (Cys); oxidation (Met) | CKNVVPLYDLLLEMLDAHR | CID | 770.06 | 3 | Lys-C, trypsin |