Figure 4.

The LPS- and CpG-responsive element in the March-I v2 promoter resides in exon 7. DCs were left untreated or were transduced with lentivirus encoding the indicated promoter construct and incubated in the presence of PBS (as a control), LPS, or CpG for 2 h. Cells were harvested, and expression of endogenous March-I v2 or GFP mRNA and GAPDH was determined by RT-PCR. GFP mRNA expression (A) or endogenous March-I v2 expression (B) in each sample was normalized to the amount of GAPDH mRNA present in the sample and expressed relative to the amount of mRNA detected in the PBS-treated control cells for each lentiviral construct as described under “Experimental procedures.” March-I RING domain primers were used to detect endogenous March-I. The results shown are the average (error bars represent S.D.) of multiple independent experiments. *, p < 0.05; ns, not significant.