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. 2018 Jan 31;293(12):4445–4455. doi: 10.1074/jbc.M117.815449

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Figure 2. — Commitment assay on rostral or caudal NPCs in hPSC differentiation. A, CHIR was added or withdrawn at the indicted time points during SD-induced neural differentiation of hPSCs. PAX6 was examined in cells from each group by FACS. SB, SB431542; DM, Dorsomorphin. B, the expression of other indicated marker genes in cells from each group were analyzed by QPCR. C, OTX2 and GBX2 were the first responders on day 2 of differentiation. **, p < 0.01; ***, p < 0.001.