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. 2018 Jan 31;293(12):4445–4455. doi: 10.1074/jbc.M117.815449

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© 2018 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 3. — OTX2 play a dominated role in neural patterning during hPSC differentiation. A, cell morphology of hESCs overexpressing OTX2, GBX2, or HOXB2. Scale bars = 100 μm. Expression levels of OTX2, GBX2, and HOXB2 were analyzed by QPCR. B, expression of OCT4 and NANOG in each indicated cell line. C, immunofluorescence results of PAX6, OCT4, and SOX2 in OTX2-overexpressing cell lines. Scale bars = 50 μm. D and E, QPCR analysis of the expression of the indicated lineage genes in wildtype hESCs and hESCs with OTX2 expression. F, expression of OTX2 and PAX6 under SD induction in GBX2 or HOXB2-overexpressing cells. **, p < 0.01; ***, p < 0.001.