Figure 5.
Analysis of the final products of HA and CS variants digested by rHCLase Er. Twenty micrograms each of HA (A), CS-A (B), CS-C (C), CS-D (D), or CS-E (E) were exhaustively digested with rHCLase Er and then separated into its constituent oligosaccharides by gel-filtration chromatography on a SuperdexTM peptide column as described under “Experimental procedures.” The elution positions of the following standard oligosaccharides are indicated by arrows: 1, disulfated CS disaccharides; 2, monosulfated CS disaccharides; 3, non-sulfated CS or HA disaccharides.