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Time-course experiments of degradation of D. gigas cartilage CS-E by rHCLase Er. CS-E (1 mg/ml) was exhaustively treated with rHCLase Er, and a 20-μg aliquot was taken at different time points for gel-filtration analysis as described under “Experimental procedures.” In an enlarged experiment, the eluted fractions of the final product from digestion for 72 h were collected as indicated by arrows for further analysis as described under “Experimental procedures.”
