Table 3.

Proteins represented differentially in OSM-treated lysosomal preparations

Sixteen (italic) and 23 (bold) proteins were differentially represented in control and OSM-treated samples (depicted in Fig. 4C). Many lysosomal enzymes were decreased by OSM, whereas the lysosomal localization of cell surface and cytoskeletal components were increased. Proteins that were absent/nearly absent from the comparator sample (Infinite (INF) or 0) are not plotted in Fig. 4F.

Protein name	Gene symbol	Accession no.	p valuea	-Fold changeb
MIT domain–containing protein 1 OS	Mitd1	Q8VDV8	0.034	0
Epoxide hydrolase 1 OS	Ephx1	Q9D379	0.015	0.06
Galactocerebrosidase OS	Galc	P54818	0.0021	0.09
Palmitoyl-protein thioesterase 1 OS	Ppt1	O88531	0.00089	0.1
Acid ceramidase OS	Asah1	Q9WV54	0.014	0.1
E3 ubiquitin-protein ligase RNF13 OS	Rnf13	O54965	0.043	0.1
CD82 antigen OS	Cd82	P40237	0.0091	0.2
β-Hexosaminidase subunit beta OS	Hexb	P20060	0.022	0.2
Lysosomal α-mannosidase OS	Man2b1	O09159	0.014	0.3
IST1 homolog OS	Ist1	Q9CX00	0.019	0.3
β-Glucuronidase OS	Gusb	P12265	0.047	0.3
Lactadherin OS	Mfge8	P21956	0.0067	0.4
Ras-related protein Rab-18 OS	Rab18	P35293	0.025	0.4
Claudin-3 OS	Cldn3	Q9Z0G9	0.011	0.5
Retinoid-inducible serine carboxypeptidase OS	Scpep1	Q920A5	0.014	0.5
Sideroflexin-1 OS	Sfxn1	Q99JR1	0.046	0.5
Lysosome-associated membrane glycoprotein 2 OS	Lamp2	P17047	0.003	1.5
Integrin β-1 OS	Itgb1	P09055	0.017	2.2
Monocarboxylate transporter 1 OS	Slc16a1	P53986	0.022	2.5
Basigin OS	Bsg	P18572	0.0017	3.1
Importin subunit β-1 OS	Kpnb1	P70168	0.0026	3.5
Annexin A1 OS	Anxa1	P10107	0.0051	4.3
ADP-ribosyl cyclase 2 OS	Bst1	Q64277	0.022	4.5
Catenin δ-1 OS	Ctnnd1	P30999	0.013	4.9
Annexin A6 OS	Anxa6	P14824	0.0025	5.8
Junction plakoglobin OS	Jup	Q02257	0.023	8.1
Talin-1 OS	Tln1	P26039	0.032	8.5
Flotillin-1 OS	Flot1	O08917	0.036	8.7
Keratin, type II cytoskeletal 8 OS	Krt8	P11679	0.0016	9.3
T-complex protein 1 subunit β OS	Cct2	P80314	0.018	9.6
Vinculin OS	Vcl	Q64727	0.027	12
Protein-glutamine γ-glutamyltransferase 2 OS	Tgm2	P21981	0.044	14
Microsomal glutathione S-transferase 1 OS	Mgst1	Q91VS7	0.0049	INF
Filamin-A OS	Flna	Q8BTM8	0.0056	INF
Integrin α-6 OS	Itga6	Q61739	0.027	INF
Annexin A11 OS	Anxa11	P97384	0.028	INF
Filamin-B OS	Flnb	Q80X90	0.041	INF
Integrin α-7 OS	Itga7	Q61738	0.041	INF
Inactive tyrosine-protein kinase 7 OS	Ptk7	Q8BKG3	0.042	INF

^a Determined by t test.

^b OSM/vehicle as measured by emPAI.