Figure 4.

Inhibition of TGase activity by cystamine does not affect level of cell wall components. Yeast cells were grown with different concentrations of cystamine (0, 50, 100, and 200 mm) for 6 h at 28 °C, and amounts of mannoproteins, glucan, and chitin were determined by flow cytometry. Chitin staining was first evaluated by fluorescence confocal microscopy using calcofluor (A). Chitin (B and C), glucan (D and E), and mannoproteins (F and G) were stained with CFW, methyl blue, and FITC-ConA, respectively. Mean fluorescence intensity was calculated for each experiment and graphed. Histograms did not show statistically significant variation in the amount of these components in cells grown at different cystamine concentrations. AutoFL, autofluorescence.