Figure 3.

Effect of laminar shear stress on deactivation properties of hERG1a. A. reversal potential measurement from a fully activated I–V. Currents were elicited by a 2-s depolarizing step potential to 0 mV followed by a 3-s repolarization step (between −120 and +20 mV). Scale bar, 2 nA and 1 s (n = 4). B and C, tail currents measured between −120 and −40 mV with an interval of 10 mV were fitted with a double-exponential function. Time constants of fast and slow components at −60 and −100 mV are plotted for comparison between pre-shear and shear stress (p < 0.05, n = 5, paired t test). Individual data points at each given voltage are represented as mean ± S.E.