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. 2018 Jan 5;293(12):4289–4303. doi: 10.1074/jbc.RA117.000432

Figure 8.

Figure 8.

Shear stress and integrin pathway. Representative current traces from hERG1a channel before (pre-shear) and during laminar shear stress (shear stress) are indicated by arrows. Currents were elicited by a depolarizing step to 0 mV followed by a repolarization to −60 mV from a holding potential of −80 mV. A, 0.1 mm RGD peptide incubated for 30 min to block integrin binding to fibronectin. Scatter plot shows complete loss of mechanosensitivity as seen in both step and tail current (mean ± S.D., p < 0.01, n = 8, paired t test) during LSS application. Scale bar, 1 nA and 500 ms. B, 50 μm FAK inhibitor 14 dialyzed in the cell through a patch pipette for 15 min. Scatter plot shows attenuation of mechanosensitivity of hERG currents in step current (mean ± S.D., p < 0.05, n = 8, paired t test) and tail current (mean ± S.D., p < 0.01, n = 8, paired t test) during application of shear stress. Scale bar, 1 nA and 500 ms. ns, non-significant.