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Pho7-binding sites in the pho84 promoter. EMSAs using 32P-labeled DNA fragments embracing the indicated sequences upstream of the pho84 transcription start site (defined as +1). The nucleotide margins of the DNA segments are indicated at the top. Reaction mixtures (10 μl) containing 0.05 pmol of labeled DNA, 340 ng of poly(dI-dC), and 0–2 ng of Pho7-DBD were incubated for 10 min at room temperature. The mixtures were analyzed by native PAGE. An autoradiograph of the gel is shown.
