Figure 5.

Reo-induced inflammatory responses eradicate HCV from cell culture and exert antiviral effects in vivo. (A) Huh7-JFH1 cells were treated for 14 days with reovirus-CM (RCM) or conditioned media (CM) derived from JHH1 cells at a dilution of 1:16, or with control media, or purified interferon (IFN)-β at 50 pg/mL. Cells were then either quantified for luciferase activity (bottom right) or seeded at the densities shown in top right and cultured in media containing G418 for 4 days before fixation and staining in methylene blue (left). (B) Representative in vivo imaging system (IVIS) pictures of HCV replicon luciferase activity within subcutaneous Huh7-JFH1 xenografts in SCID mice. Mice were treated with twice weekly Itu injections of PBS or 1×10⁵ PFU uv-Reo, starting immediately following baseline assessment. (C) (Left) Quantification of IVIS luciferase intensity from (B). Graph shows per cent luciferase activity in relation to day 0 post-treatment baseline values. (Right) Tumour volume of xenografts shown in (B).