Table 1.

Summary of studies on miRNAs of Neotropical and other fish species with relevance on aquaculture.

Group	Species	Tissue	Methodology	References
Neotropical	Tambaqui	Liver and skin	Illumina sequencing technology and functional annotation	Gomes et al., 2017
	Pacu	Skeletal muscle	Real-time PCR	Paula et al., 2017
	Midas cichlids	Not informed by authors	Target prediction and Illumina sequencing technology	Franchini et al., 2016
Others	Nile tilapia	embryo	RNA-seq high-throughput sequencing and miRNA target prediction analysis	Eshel et al., 2014
		Gonads	Solexa sequencing with real-time PCR expression, miRNA target prediction analysis and miRNA pathways analysis	Wang et al., 2016
			Solexa sequencing with real-time PCR expression	Xiao et al., 2014
		Kidney	Real-time PCR and luciferase reporter assay	Yan et al., 2012b,c; Zhao et al., 2016
		Skeletal muscle	Prediction of MyoD-binding miRNAs, real-time PCR and luciferase reporter assay	Khvorova et al., 2003; Yan et al., 2013b
			Microarray and luciferase reporter assay	Kim et al., 2006
			Silencing of miR-206 in vivo using the antagomir method	Yan et al., 2013b
	Atlantic Salmon	Blood	RNA-seq high-throughput sequencing	Kure et al., 2013
	Rainbow trout	Larvae	Real-time PCR	Ramachandra et al., 2008
		Liver	Prediction of miRNA targer gene and real-time PCR	Mennigen et al., 2012
		Muscle	RNA-seq high-throughput sequencing and target prediction	Salem et al., 2010
	Grass carp	Blood	Solexa sequencing technology, real-time PCR, directional cloning and induction of expression of three immune factors in vitro.	Xu et al., 2016
		Embryo	Real-time PCR	Xu et al., 2014
		Spleen	Solexa sequencing technology, real-time PCR, target prediction and integration with mRNA expression and luciferase report system	Xu et al., 2015