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. 2018 Mar 19;9:508. doi: 10.3389/fimmu.2018.00508

Figure 1.

Figure 1

THP-1 cells, routinely grown in the presence of 1 mM extracellular arginine, were transfected with scrambled or SLC7A7 short interference RNA (siRNA) in the presence of 80 nM phorbol-12-myristate-13-acetate (PMA), as described in Section “Materials and Methods”; after 96 h, cells were analyzed for the expression of SLC7A7 mRNA with RT-qpolymerase chain reaction (RT-qPCR) (A) and for intracellular arginine content by means of HPLC/ESI-MS-MS (B). In the same cells, the production and release of IL1β and TNFα were monitored with RT-qPCR (C) and ELISA assay (D), respectively. Data are mean ± SEM of four different determinations, each performed in duplicate. *p < 0.05, **p < 0.01, ***p < 0.001 vs. scrambled siRNA with One sample t-test (A,C) or Student’s t-test for paired samples (B,D).