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. 2018 Mar 19;9:508. doi: 10.3389/fimmu.2018.00508

Figure 2.

Figure 2

THP-1 cells were grown in the presence of the indicated concentrations of extracellular arginine. (A–C) Cells were differentiated to macrophages by exposure to 80 nM phorbol-12-myristate-13-acetate (PMA) for 96 h, then arginine content was measured by means of HPLC/ESI-MS-MS (A), while the expression of IL1β (B) and TNFα (C) mRNAs was monitored with RT-qpolymerase chain reaction (RT-qPCR). Data are mean ± SEM of five independent determinations. *** p < 0.001 vs. [Arg]extracellular 0.1 mM. (D–F) Cells were transfected with scrambled or SLC7A7 short interference RNA (siRNA) along the PMA-induced differentiation, as described in Section “Materials and Methods,” and analyzed for the expression of SLC7A7 (D), IL1β (E), and TNFα (F) mRNAs with RT-qPCR. Data are mean ± SEM of four different determinations, each performed in duplicate. *p < 0.05, **p < 0.01, ***p < 0.001 vs. scrambled siRNA with one-way ANOVA followed by Bonferroni post hoc test.