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. Author manuscript; available in PMC: 2018 Mar 26.
Published in final edited form as: Arch Biochem Biophys. 2016 Oct 15;612:35–45. doi: 10.1016/j.abb.2016.10.008

Figure 7. Kinetic response of USP17 at varying pH.

Figure 7

The activity of USP17 was measured with Ub-Rho110 from pH 6 to 10.5 with 6.25 μM USP17. Two concentrations of Ub-Rho110 surrounding the Km value were tested, 0.5 μM and 5 μM. Assays were performed in triplicate. Curves were fit to a bell-shaped rate profile as described in the Material and Methods.