Table 7.

Demodex detection rate of PCR and FP analyses compared with the detection rate of DSS in a subset of subjects

Gel lanea	Case IDb	Sample type	PCRa	FP–IS	FP–CV	DSS–IS	DSS–CV
1	17	Frozen, whole skin	+	+	+	+	+
2	18	Frozen, whole skin	+	+	−	+	+
3	42	Formalin-fixed, whole skin	+	+	+	+	+
4	43	Formalin-fixed, whole skin	+	−	+	+	+
DNSa	44a	Formalin-fixed, whole skin	+	+	−	+	+
DNS	45a	Formalin-fixed, whole skin	+	+	−	+	+
5	46	Formalin-fixed, whole skin	+	+	+	+	+
6	46	Individual mite
7	20	Individual mite	+	−	+	+	+
		Detection rate (%)c	100%	75%	62.5%	100%	100%

CD, caudal dorsum; CV, caudal ventrum; DNS: data not shown; DSS, deep skin scrape; FP, fur pluck; IS, interscapular region; SSS, superficial skin scrape; TI, tape impression

^aGel lane indicated corresponds to the gel shown in Figure 3. Results shown in the table are combined from multiple PCR assays. Samples 44 and 45 were run on another gel (not shown).

^bCase ID, for cross-referencing, refers to the animal identification number used in a previous publication.52

^cDetection rates were compared between tests by using McNemar's test. None of the values, including those for FP–IS and FP–CV, differed significantly from the DSS–IS or DSS–CV detection rate.