Table 1.
Results of acetic acid testing in frogs (n = 6 per group)
MS222 | MS222 + FM | Etomidate | Etomidate + FM | Benzocaine | Benzocaine + FM | |||
1 h | 1 | 2 | 0 | 0 | 0 | 4 | ||
3 h | 2 | 2 | 1 | 0 | 1 | 4 | ||
6 h | 4 | 2 | 0 | 1 | 0 | 4 | ||
24 h | 2 | 2 | 1 | 4 | 1 | 5 |
Frogs were anesthetized with either MS222, etomidate (22.5 mg/L), or benzocaine (0.1%) and subsequently did or did not receive flunixin meglumine (FM; 25 mg/kg) in the right dorsal lymph sac. Data are reported as the number of animals that did not react when ≥10% acetic acid was applied to the back legs at the indicated time points.