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. 2018 Feb 2;39(4):1591–1600. doi: 10.3892/or.2018.6245

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Copyright: © Sun et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — XIST promotes PC cell growth in vitro. (A) Expression level of lncRNA-XIST was detected by qRT-PCR assay in human pancreatic ductal epithelial (HPDE) cells and PC cell lines (PANC-1, ASPC-1, MIA PaCa-2, HPAC, CFAPC-1 and BxPC-3). Each assay was performed for at least three biological replicates (*P<0.05, **P<0.01, ***P<0.001). (B) XIST expression was examined by qRT-PCR assay in BxPC-3 cells transfected with XIST plasmid and control, and PANC-1 cells transfected with shXIST and control. Relative expression was normalized to GAPDH expression (**P<0.01). (C) MTT assay was performed to measure the viability of BxPC-3 and PANC-1 cells treated as in B (**P<0.01). (D) Colony formation assay was used to detect the proliferation ability of the transfected cells. (E) Cell Cycle distribution was analyzed by flow cytometry in BxPC-3 and PANC-1 cells after transfection (**P<0.01, ***P<0.001). PC, pancreatic cancer; lncRNA, long non-coding RNA; XIST, X inactive-specific transcript.