Figure 6.

miR-34a-5p abrogates the facilitation of malignant behavior mediated by XIST. (A) The correlation between miR-34a-5p and XIST was determined by Pearson's correlation coefficient in clinical samples (R²=0.2175, P<0.001). (B) Luciferase reporter assay was performed in 293T cells co-transfected with luciferase construct (pGL3-Control, pGL3-XIST WT or pGL3-XIST-Mut) and negative control (miR-control) or miR-34a-5p mimics, respectively (***P<0.001). (C) BxPC-3 cells were transfected with control, XIST, XIST plus miR-NC or XIST plus miR-34a-5p mimics; PANC-1 cells were transfected with control, shXIST, shXIST plus miR-NC or shXIST plus miR-34a-5p inhibitors, respectively. qRT-PCR assay was used to detect miR-34a-5p expression. GAPDH was used as control (**P<0.01, ***P<0.001). (D) MTT assay was performed to determine the viability of BxPC-3 and PANC-1 cells treated as in C. (E) The migration and invasion abilities were detected by Transwell assays in BxPC-3 and PANC-1 cells treated as in C (***P<0.001). XIST, X inactive-specific transcript;