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. 2018 Feb 12;39(4):1649–1657. doi: 10.3892/or.2018.6259

Figure 3.

Figure 3.

ITGB8 is a direct target of miR-199a-3p. (A) Sequence alignment of two putative miR-199a-3p binding sites in the 3′-UTR of ITGB8. (B) The protein levels of ITGB8 in SKOV3/CDDP and SKOV3 cells examined by western blotting. (C) Relative luciferase activities of plasmids carrying ITGB8 3′-UTR-1 or ITGB8 3′-UTR-2, or both in SKOV3/CDDP cells co-transfected with miR-199a-3p mimics. Data shown are mean ± SD, ***P<0.001, compared with control mimic (one-way ANOVA with Dunett's test). (D) Relative luciferase activities of plasmids carrying WT or mutant of ITGB8 3′-UTR-2 in SKOV3/CDDP cells co-transfected with miR-199a-3p mimics or miR-199a-3p inhibitor. Data shown are mean ± SD, ***P<0.01, compared with inhibitor (one-way ANOVA with Dunett's test). (E) The expression levels of ITGB8 in SKOV3/CDDP cells were examined by real-time PCR and western blotting. Data shown are mean ± SD, P<0.01, compared with miR-NC (Student's t-test).