Figure 3.

APR-246 increases DNA binding of mtp53 in BT-474 breast cancer cells.

Notes: Cells were grown overnight in DMEM/F12 medium supplemented with 5% FBS. Cells were then washed once with PBS and treated with 50 μM APR-246 for 1 hour, prior to harvest by scraping and subsequent preparation of nuclear extracts. TransAM assays were performed using nuclear extracts (2.5 μg), with each sample being analyzed in triplicate. Activation of DNA-binding ability was compared with the vehicle-treated group (control), whose value was set at 1. Nuclear extract of wtp53-expressing MCF-7 cells (provided with the TransAM kit) was used as a positive control (Positive-C). Data are shown as the mean±SEM from three different determinations. *Significantly different from untreated controls (P<0.05; ANOVA).

Abbreviations: ANOVA, analysis of variance; FBS, fetal bovine serum; mtp53, mutated form of p53; SEM, standard error of the mean.