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. 2018 Mar 22;13:1819–1829. doi: 10.2147/IJN.S152976

Figure 7.

Figure 7

Detection of EPCs labeled with multimodal imaging probes in ischemic hindlimbs. (A) Cy5.5 and random signals from probe-labeled EPCs were colocalized in the injured tissue stained with fluorescent Alexa488-CD34, CD133, VEGF receptor 2 and CXCR4 (EPC markers). Probe-labeled EPCs positive for EPC markers migrated into microvessel walls 3 days after transplantation. Cy5.5 and EPC antibody positive cells are shown by arrows. The scale bars are 30 μm. (B) The numbers of Cy5.5-positive and rhodamine double-positive EPCs per square millimeter within the ischemic region were recorded at 3 days after the induction of injury. (C) Western blot showing increased VEGF receptor 2, VEGF, CXCR4 and SDF-1α levels in the ischemic muscle of EPC-treated mice on days 1 and 3 compared with the controls. (D) and (E) Bottom panel shows the results of quantification of the bands. *P<0.05, ***P<0.001.

Abbreviations: EPC, endothelial progenitor cell; VEGF, vascular endothelial growth factor; CXCR4, C-X-C chemokine receptor 4; SDF-1α, stromal cell-derived factor-1alpha; rho, rhodamine; DAPI, 4′,6-diamidino-2-phenylindole; VEGFR2, VEGF receptor 2.