Figure 4.

Generation of Functional mCherry-Tagged CDKA1 and CDKB1.

(A) DNA used for transformation. The native sequences, including promoter, terminator, and introns were used for both CDKA1 and CDKB1. The sequence encoding the mCherry tag was added at the 5′ end of the coding sequence following a 3x GGGGS linker sequence. Paromomycin was used to select for transformants.

(B) Immunoblot showing expression of the tagged CDK transgenes in random transformants. AtpB was used as a loading control.

(C) Complementation of temperature sensitivity of cdka1-1 csl89-1 and cdkb1-1 in the transformants from (B).