Figure 5.

Regulation of CDKA1 Abundance and Kinase Activity.

Strains of the indicated genotypes were synchronized by nitrogen deprivation and released at 33°C. The contrast was adjusted such that images can be compared within but not between subfigures.

(A) Immunoblot detection of CDKA1-mCherry.

(B) Kinase activity of immunoprecipitated CDKA1-mCherry on a histone H1 substrate. Note: Background kinase activity in immunoprecipitates from untagged cells was low and varied little through the cell cycle (data not shown).

(C) Quantification of (A) and (B) and the fraction of cells/cell clusters with ≥2C DNA content as determined by flow cytometry.

(D) and (E) Cyclin dependence of CDKA1-associated kinase activity. See also Supplemental Figure 7A.

(F) and (G) CDKB1 and APC negatively regulate CDKA1-associated kinase activity.