Figure 7.

Regulation of CDKB1 Abundance and Associated Kinase Activity

Strains of the indicated genotypes were synchronized by nitrogen deprivation and released at 33°C. Contrast was adjusted such that images can be compared within but not between subfigures.

(A) Immunoblot detection of CDKB1-mCherry.

(B) Quantification of (A) and fraction of cells with ≥2C DNA content as determined by flow cytometry.

(C) and (D) Cyclin dependence of CDKB1-associated kinase activity. See also Supplemental Figure 7B.

(E) and (F) APC negatively regulates CDKB1-associated kinase activity. Note: Background kinase activity in immunoprecipitates from untagged cells was low and varied little through the cell cycle (data not shown).