Figure 8.

CDKB1 Is a Nuclear Protein during Division Cycles.

Confocal micrographs from a population of live cells expressing CDKB1:mCherry and ble-GFP as a nuclear marker (Y. Li et al., 2016). All images are average intensity z-projections. Contrast was adjusted separately for each trans and nuclear (GFP) image for clarity. CDKB1 images are contrast adjusted to the maximum of the group, so intensities can be compared. The non-nuclear signal in the mCherry channel was observed at similar levels in control strains lacking the transgene (data not shown).

(A) Newborn cell with no detectable CDKB1.

(B) to (E) The 1-, 2-, 4-, and 8-cell clusters, respectively, with CDKB1 in all nuclei. Bars = 10 μm.

(F) The 16-cell cluster of postmitotic newborn cells (just before hatching) lack CDKB1.

(G) Nuclear concentration of CDKB1 as a function of number of cells per cluster (see Supplemental Methods). Bar = 10 μm.