Figure 6.

Heterogenous Effector Gene Expression in Fungal Aggregates.

Maize seedlings were infected with mixtures of FB1 carrying the indicated reporter constructs (top) and FB2 wild-type strains. Seven days postinoculation, fungal aggregates within the tumor tissue were visualized by confocal microscopy. The fluorescence of GFP and mCherry was monitored and merged with the respective bright-field (BF) image. All images are projections of multiple z-stacks. GFP fluorescence indicative of effector gene expression was mainly detected at the surface of the aggregates, while mCherry control fluorescence was rather evenly distributed throughout the aggregates.