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. 2018 Jan 24;30(2):397–414. doi: 10.1105/tpc.17.00420

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© 2018 American Society of Plant Biologists. All rights reserved.

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Figure 9. — Nodules Induced by S. meliloti 2011 Mutants Producing Modified NFs (20 dpi).

M. truncatula R108 wild-type and nfh1-3 mutant plants were inoculated with S. meliloti strains that produce different NFs.

(A) Photographs of nodules at the time of harvest (20 dpi). Bars = 2 mm.

(B) Quantification of different types of nodules. Only elongate nodules were observed for wild-type plants. The nfh1-3 mutant formed elongate, bifurcate and palmate-coralloid nodules. Data indicate means (±se) based on analysis of 10 plants of each genotype.

GFP, strain 2011 carrying pHC60 constitutively expressing GFP; nodL, strain 2011nodL::Tn5-GFP producing NFs without O-acetyl group at the nonreducing end; nodFE, strain 2011ΔnodFE-GFP producing NFs with vaccenic acid; nodFnodL, strain ΔnodFnodL::Tn5-GFP producing NFs with vaccenic acid and lacking an O-acetyl group.