Figure 7.

AG Regulates Cytokinin Activity.

(A) to (D) Expression of ProTCSn:GFP (green) in the inflorescences of Pro35S:AG-GR ag-1 under DMSO (A) and DEX (B) treatment and Pro35S:AG-GR ag-1 arf3-29 under DMSO (C) and DEX (D) treatment for 4 d.

(E) GFP transcript levels in inflorescences of the indicated plants. Inflorescences containing stage 6 and younger flowers were collected for RT-qPCR.

(F) to (H) Representative silique phenotypes of ag-11 (F), ag-11 ipt3 ipt5 ipt7 (G), and ag-11 ahk4 (H). The leftmost silique has one carpel of the primary gynoecium removed to show the structures contained therein. ag-11 produces short and bulged siliques with additional tissues growing inside. ipt3 ipt5 ipt7 and ahk4 largely rescued the severe FM determinacy defect of ag-11.

(I) and (J) Longitudinal sections of representative ag-1 (I) and ag-1 ipt3 ipt5 ipt7 (J) flowers at similar developmental stages. The FM size (marked by red lines) of ag-1 ipt3 ipt5 ipt7 is smaller than that of ag-1.

(K) Quantification of FM size (µm) in ag-1 (n = 15) and ag-1 ipt3 ipt5 ipt7 (n = 12). **P < 0.01 (Student’s t test).

(L) Time-course expression analysis of the indicated genes in Ler and Pro35S:AG-GR ag-1 inflorescences under DEX treatment. Ler inflorescences containing stage 6 and younger flowers and Pro35S:AG-GR ag-1 inflorescences containing unopened flowers were collected for RT-qPCR.

(M) IPT5 expression in inflorescences of the indicated plants. RNA was extracted from Ler and arf3-29 inflorescences containing stage 6 and younger flowers for RT-qPCR. Pro35S:AG-GR ag-1 and Pro35S:AG-GR ag-1 arf3-29 were treated with DEX or DMSO for 4 d, and inflorescences containing unopened flowers were collected for RT-qPCR.

Error bars in (E), (L), and (M) indicate the sd from three biological replicates with independently prepared inflorescence materials. *P < 0.05 and **P < 0.01 (Student’s t test). Bars = 50 µm in (A) to (D), (I), and (J) and 1 mm in (F) to (H).